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Since nicotinamide nucleotide transhydrogenase (NNT), which is functional in C57BL/6N mice but not in C57BL/6J mice (Freeman et al., 2006), oxidizes mitochondrial matrix NADH to NAD + , the increased longevity of the C57BL/6J strain may be due in part to the reduction of the mitochondrial matrix [NAD + ]/[NADH]