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Once colonies were found, competent cells were prepared and transformed with a URA resistance cassette with homology arms to endogenous CHC1 gene (See Supplementary Table 5 for primer S2/S3 sequence) using the established PCR cassette protocol (pFA6a-KlURA3 Vector) 65

Ethics The Institutional Review Board of each participant hospital, including Seoul St

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However, sulfur metabolism impairment can act as a significant contributor to histamine intolerance , and a unique connection exists between the two

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