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An enema filled with coffee

A 2% inoculum (2 mL in a 100 mL broth culture) of this culture was then transferred into 100 mL TSB and incubated for 24 h at 37 C

Study approvals were obtained from the National Cancer Centre Ethics Review Committee (Singapore), American University of Beirut Institutional Review Board (Lebanon) and South and West MultiCentre Research Ethics Committee (MREC 00/6/69

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