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Dai YL, Luk TH, Yiu KH, Wang M, Yip PM, et al

For analysis of flavonoids by HPLCMS, reaction volumes were split after the plate reader incubations, and a 40 l aliquot was extracted with 80 l of 75% methanol in H 2 O, 1 mM EDTA, 0.3% hydrochloric acid for quantification of cyanidin ( 8 ) concentration while a second 40 L aliquot was extracted with 80 l of 75% acetonitrile in H 2 O, 1 mM EDTA for quantification of all other flavonoids