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Validation of process optimization The optimal preparation conditions for rhCol III-LIPS were obtained using Design Expert 8.0.6 software with encapsulation efficiency as an indicator, which are as follows: a rhCol III-to-lipid ratio of 1:52.41, a membrane-material ratio of 4.31:1, an ultrasound time of 20.10 min, and an encapsulation efficiency of 91.89%

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doi: 10.1038/s41467-01908759-0 44 ZhangZLiuRShuaiYHuangYJinRWangXet al