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The scRNA/CITE-seq libraries were prepared using the GemCode Single Cell 3 Gel Bead and Library kit, version 3 (10x Genomics 1000128) according to the manufacturers instructions with the addition of amplification primer (3 nM, 5CCTTGGCACCCGAGAATT*C*C) during cDNA amplification to enrich the TotalSeq-A cell surface protein oligos

Buffer was drained and the reaction quenched by washing beads twice for 5 min with 100 mM Tris, pH 8.0

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1d, e), indicating that LOsG-impaired -cell function and GT

These conflicting results may reflect context-dependent actions of LCN2 in bone and/or differences in the underlying genetic models