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HEK293 platA cells were transfected with pMXs-CIP4-mEos4b-IRES-HygR or pMXs-CIP4-mCherry-IRES-HygR using 293-fectin (Invitrogen) for 2 days to produce retrovirus, and the supernatant containing the retrovirus was filtered and infected with CIP4 KO PANC-1 cells in the presence of polybrene (Sigma, TR-1003) at 32 g/ml

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