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Complex I activity was assayed kinetically by measuring the Vmax reduction of 2,6-dichlorophenol-indophenol (DCPIP) by spectrophotometer (Specter MAX 190, Molecular Devices, Sunnyvale, CA, USA) [18]

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Mistake 5: Storing at wrong temperature Reconstituted peptides must be refrigerated

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Viswanathan CT, Bansal S, Booth B et al (2007) Quantitative bioanalytical methods validation and implementation: best practices for chromatographic and ligand binding assays