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Huang L, Kinnucan E, Wang G, Beaudenon S, Howley PM, Huibregtse JM et al

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This contributes to healthier aging, maintenance of youthful vitality, and overall immune health

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After deparaffinization, sections were heated in an autoclave at 121 C for 5 min in 10 mM sodium citrate buffer (pH 6.0) or reacted with protease solution (Nichirei Biosciences, Tokyo, Japan) for epitope retrieval, after which endogenous peroxidase was inactivated by 0.3% hydrogen peroxide in methanol