2.8 Pull-down experiments Chloroplast extracts (100 g of Chl) were solubilized with 1% (w/v) dodecyl maltoside (DM) for 10 min at 4C, and incubated with 50 g of either purified His-TRX-f1, His-TRX-m1 and His-NTRC as bait proteins in binding buffer (BF, 25 mM Tris-HCl [pH 7.8], 150 mM NaCl, 5 mM MgCl 2 , 10% [v/v], glycerol, and cOmplete protease inhibitor [Roche]) overnight at 4C and 45 rpm
How fast does N-Acetyl Epitalon Amidate work
Regulation of IGF-I receptor signaling in diabetic cardiac muscle: dysregulation of cytosolic and mitochondria HSP60
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[DOI] [PubMed] [Google Scholar] 56.Chen Y.J., Lu C.T., Lee T.Y., Chen Y.J