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Speaker: Dr Paola Pellegrini (XJTLU - Xi'an Jiaotong-Liverpool University) 89 Performance Analysis of 15-Minute City Zones Through Spatial and Machine Learning Techniques With the significant growth of urban populations, cities are expanding rapidly, making urban sprawl a critical global challenge that necessitates urgent attention in policy agendas

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The day before the assay, frozen aliquots of the above-mentioned BHK cell lines were thawed, washed once in PBS (Gibco, #14190-094) and seeded in 40 L medium in a white 384-well culture plate (PerkinElmer, #6007688) at a cell density of 4000 cells/well and incubated in plastic bags over night at 37 C and 5% CO 2