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After their nucleotide sequences were confirmed, the coding regions of AsGGT1 , AsGGT2 , and AsGGT3 were cut out as Kpn I- Xho I fragments and were inserted between the Kpn I and Xho I sites in the yeast expression vector pYES2 (Life Technologies)

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3.1 Genetic factors in the pathogenesis of vitiligo The earliest evidence implicating genetic factors in the pathogenesis of vitiligo was reported by Nath et al., in 1994 (Spritz and Andersen, 2017)