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Allelic exchange substrates (AES) containing the Kan R flanked by ~1 kb identity arms for site-specific recombination were obtained by high fidelity PCR using BAC Rv412- cobM,K ::Kan R as template and Conf-KO BAC cobMK-Fw/Conf-KO BAC cobMK-Rv primers

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doi: 10.2337/diabetes.51.12.3368

rotating injection sites prevents localised irritation