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This concentration is approximately an order of magnitude higher than the in vitro IC50 of 613 M for hepatocytes, taking into account the rapid distribution and metabolism occurring in vivo

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Spectrophotometric characterisation of the Cu(II) centre, stability studies across buffer compositions, competition experiments against other chelators, and method development for HPLC or ICP analysis all consume modest amounts of material while depending heavily on the complex not having degraded in storage