Growth hormone overexpression in the central nervous system results in hyperphagia-induced obesity associated with insulin resistance and dyslipidemia
Vitamin D, acting as a neurosteroid hormone, may have a regulatory function in neurotransmission, neuroprotection, and neuroimmunomodulation [18]
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doi: 10.3389/fnut.2024.1406733 202 KikutJKoneckaNZitekMKulpaDSzczukoM
Tissue culture media supernatants from the final 24 hours of culture were collected and assayed by Amplex Red Glucose/Glucose Oxidase Assay Kit (Life Technologies, Gaithersburg, MD, USA), Lactate Assay kit (Sigma-Aldrich) and Rat Ultrasensitive Insulin ELISA (Mercodia, Uppsala, Sweden) to measure total glucose, lactate and insulin respectively