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In the STEP and SURMOUNT trials for semaglutide and tirzepatide respectively, weight loss curves generally began to flatten between weeks 36 and 60, reflecting the physiological set point adaptation that occurs as appetite suppression becomes partially offset by metabolic adaptation

For example, a 10 mg vial reconstituted with 1 mL of bacteriostatic water produces 10,000 mcg/mL

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As a result, sample pretreatment after hydrolysis is often required to effectively reduce interference from the matrix