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Germ-free C57BL/6J mice ( n = 4) were used to test the direct role of cyanocobalamin at the 40 g/ml dose to corroborate the change in colonic inflammation markers found in the conventional mice

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5-Amino-1MQ operates as an inhibitor of nicotinamide N-methyltransferase (NNMT), an enzyme central to regulating nicotinamide metabolism and intracellular NAD+ levels