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The potential cancerogenic property of insulin is among the safety concerns related with long-term insulin therapy

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Mice were acclimatized to the animal facility for 1 week before they received injections of MCF-7:2A human breast cancer cells: 2 10 7 cells were resuspended in 100 L PBS (Collaborative Biomedical Products, Bedford, MA, USA) and were bilaterally injected into the mammary fat pads of 20 ovariectomized mice

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Exclusion criteria involved studies evaluating GLP-1R agonists other than those specified