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All statistical analyses were conducted using R software (version 4.3.1), and a P value less than 0.05 was deemed as statistically significant for all two-tailed tests

Neurotransmitter agonists/antagonists, including glutamate (Sigma, G3291, USA), NMDA receptor antagonist, 2-amino-5-phosphonovaleric acid (APV) (Sigma, A8054, USA), AMPA/kainate receptor antagonist 6-Cyano-7-nitroquinoxaline-2, 3-dione (CNQX) (Sigma, C127, USA), GABA (Sigma, A2129, USA), GABA-A receptor antagonist and Bicuculline (Bic) (Sigma, 285269, USA), were dissolved in CSF tracer before use at a concentration of 0.2 mM

This has resulted in these two types of drugs being actively recommended as first-line agents in patients with diabetes with established atherosclerotic cardiovascular disease (ASCVD) [1]

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Moreover, Choline is essential for nerve function, liver performance, and metabolic processes, improving cognitive abilities