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The infected cells were subsequently resuspended in lysis buffer (containing 50 mM Tris, 10% sucrose, 50 M ATP, 1 mM phenylmethylsulfonyl fluoride (PMSF), 5 mM dithiothreitol (DTT), 1% NP40, 10 mM imidazole, 1 protease inhibitor and phosphatase inhibitor, pH 7.0, with HCl)

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