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For MS/MS, the following settings are used: isolation window width 1.2 m/z with an offset of 0.5 m/z, stepped NCE at 10, 15, and 25 a.u., minimum AGC 5E 2 , and dynamic exclusion of previously sampled peaks for 8 s

The brain tissues were rinsed with ice-cold PBS (pH 7.4) to thoroughly remove excess blood and then weighed before homogenization

The Need for Carnitine Much like creatine, L-carnitine can be made in many tissues, so healthy people tend to have enough L-carnitine

This hormone signals the brain to reduce hunger, slows gastric emptying to keep food in the stomach longer, and enhances insulin secretion in a glucose-dependent manner