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Additional genetic polymorphisms occurring in choline and one-carbon metabolic pathways may alter the dietary requirement for choline and thus increase the likelihood of developing signs of deficiency when choline intake is inadequate (9, 20, 21)

Within the gastrointestinal system, it has been investigated for its role in preserving epithelial continuity and local perfusion

DM003-R500) and 5 l of the amplified PCR product were loaded in each agarose well, which was then placed in 1X TAE buffer (1%) and operated at 100 V for about two hours

Acknowledgements Authors are grateful to Panjab University, Chandigarh, India for facilities

The TmGst2 and TmGst10 showed high expression levels in conidia