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pH 7.8) and plated onto Brain Hearth Infusion (BHI) agar plates supplemented with 5% sheep blood

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Search terms included combinations of the following Medical Subject Headings (MeSH) and keywords: riboflavin OR vitamin B2 OR flavin adenine dinucleotide OR flavin mononucleotide AND neurological disease OR nervous system disorder OR brain disease AND mitochondria OR oxidative stress OR inflammation OR gene therapy OR riboflavin transporter deficiency OR disease-specific terms (e.g., migraine, Parkinsons disease, multiple sclerosis, MADD, BVVL)

Additionally, the vascular endothelium (the inner lining of the blood vessels) requires significant energy to produce nitric oxide, a molecule essential for regulating blood vessel dilation and constriction