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Moreover, TMAO was found to affect intracellular calcium handling and contractility of rat ventricular cardiomyocytes in vitro (Savi et al., 2018)

In this study, the intestinal microenvironment was recapitulated by applying defined mechanical deformations and fluid hydrodynamics, exploiting a coculture with a host microbiome while allowing the real-time monitoring of cell morphology and cell-cell interactions, in a manner not possible with a standard organoid culture

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