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24 hours for beef bones [20] Prefer beef bones over chicken for higher amino acid content [22] Use long/marrow-containing bones rather than small bones [22] Do not remove fat until after straining (retains more amino acids) [22] Roast bones first at 400-450 degrees F for 30-60 minutes for deeper flavor [48] Storage Most bone broths need only be refrigerated after opening

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While most individuals who consume balanced diets have ample B12, factors including stress and poor eating habits can lead to a deficiency