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GSH depletion causes oxidative stress and lipid peroxidation To evaluate the downstream effects of GSH depletion, we measured soluble ROS in RPE cells treated with Cys 2 starvation, BSO, or erastin by flow cytometry using the fluorescent probe H 2 DCFDA

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FIGURE 8 Recommendation: Based on the current evidence and expert opinions, NAC cannot be recommended as a standard therapy in ILDs

Although many synthetic antioxidants such as butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) are widely used in the world, natural antioxidants are highly sought after at home and abroad for their possible greater safety and healthpromoting effect (Liu et al., 2015