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Additional risk factors include advancing age (due to reduced gastric acid production), excessive alcohol consumption (impairing absorption and storage), and rare genetic disorders affecting B12 transport or metabolism

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Blood was collected from the medial saphenous vein for the measurement of erythrocyte glutathione profiles, leucocyte GSH, plasma and erythrocyte AA profiles, mitogen-induced lymphoproliferative response, serum amyloid A (SAA), 8-hydroxy-2'-deoxyguanosine (8-OHdG), one of the major products of DNA oxidation widely used as a biomarker of oxidative stress (Reference Mecocci, Fan and Fulle31) , and biochemistry and haematology parameters (Fig