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The messenger RNA expression levels of the (A) SREBP-2, (B) ACAT1, (C) HMGCR, (D) SQLE, (E) LXR, (F) CYP7A1, (G) LDLR, and (H) PCSK9 genes were analyzed with quantitative real-time polymerase chain reaction and normalized to that of the ACTB gene

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We also observed increased tissue weights for heart and kidneys in female but not male SKO mice, consistent with previously reported organomegaly in this model ( Supplementary Figures S1C, D ) Finally, blood glucose levels were examined in the non-fasted state before and after 10 days of treatment