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GPX was measured adding cell extract to 50 mmol/l potassium phosphate pH 7.0 containing 1 mM EDTA, 1.5 mM sodium azide, 0.15 IU of purified glutathione reductase from bakers' yeast, 0.45 mM GSH, 0.2 mM NADPH, 1.2 mM cumene hydroperoxide One unit of GPX was defined as the amount of enzyme which produces 1 mol of GSSG (oxidized glutathione)/min at 25C

Because the peptide was still shown to be effective 14 days post-injury, the researchers suggested that there is increased activity of wound healing mechanisms to increase mechanical strength, as well as an increase in potentiated activity that could lead to beneficial results

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Grooms SY, Noh KM, Regis R, Bassell GJ, Bryan MK, Carroll RC et al (2006)