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Aliquots at the various time points after fecal slurry inoculation were collected and acidified with 2 mL 0.1N HCl (Sigma-Aldrich, 50%/50% v/v), centrifuged at 4 C at 900 g for 8 min, filter-sterilized in a 0.22-m cellulose acetate filter, and frozen at 80 C until performing the experiments for the determination of the amount of TMA in the fecal slurry supernatants

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Withers GD, Silburn SR, Forbes DA

The dose escalation schedule spans 16 to 20 weeks: 0.25 mg for four weeks, 0.5 mg for four weeks, 1.0 mg for four weeks, 1.7 mg for four weeks, and finally 2.4 mg as the maintenance dose