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One main finding on the structural side is that the glutathione-hosting site is highly conserved, and uses the negatively charged carboxylate functions of the glycine and glutamic acid residues of glutathione as anchors for substrate recognition by positively charged amino acid residues, as well as negatively charged residues to hold the protonated amino group of the glutamic acid residue

This is less common and typically reserved for short-term, highly targeted research applications where a maximum effect is being studied

The deltoid and gluteal sites remain standard because they offer ease of access, predictable muscle depth, and lower risk of nerve or vessel injury

Mutant p53 may not only lose the tumor suppressor function of wild-type p53 but also acquire new tumor-promoting functions, such as promoting tumor invasion, metastasis, and drug resistance