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Participants were randomised (1:1) to 6-months therapy post-LAGB, with further 6 months off-treatment follow-up

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Amplification cycles were: initial denaturation at 94 C for 3 min, followed by 35 cycles of denaturation at 94 C for 30 s, annealing at 55 C for 30 s, and extension at 72 C for 1 min, followed by a final extension at 72 C for 7 min

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