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Conjugation efficiency, regioselectivity and functional integrity of antigen-presenting PfFt nanoparticles Antigen-presenting PfFt nanoparticles, i.e ., Gn-Y-PfFt and RBD-Y-PfFt, were analyzed by mass spectrometry (MS) to precisely estimate the molecular weight of the monomeric conjugates, determine the ratio of functionalized to unfunctionalized PfFt monomers, and evaluate whether covalent bonding occurred specifically at the mutant cysteines or other native amino acids on PfFt