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When COMT is slow, more 4-OHE2 remains available for oxidation to reactive quinones increasing demand on GSTM1/GSTT1 glutathione conjugation at exactly the point where their absence is most consequential

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These in-organ changes in energy metabolism are also reflected in the plasma metabolome after ischemic AKI, which is characterized by the depletion of numerous amino acids and energy substrates 6

Image J software was used to perform a densitometric analysis of the blots