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In the study, they observed that Ex-4 treatment activated the Phospho- Akt/Phospho- endothelial nitric oxide synthase (p-Akt/p-eNOS) signaling pathway after MCAO, suggesting the recruitment of signal pathways which can protect against oxidative stress

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By contrast, changes in the distal radius, a non-weight bearing skeletal site, were not observed

Creatinine was measured using enzymatic, colorimetric methods and albumin using immunoturbidimetric methods in the first morning urine sample to calculate the albumin-creatinine ratio (ACR)