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See the protocol for the details of the downstream analysis

Factors contributing to bleeding during detoxing include pre-existing medical conditions, medications, nutrient deficiencies, and changes in blood pressure or the body's response to environmental factors

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Drinking on an empty stomach raises both intoxication and the risk of low blood sugar, which the medication can make harder to predict

Also, NPY KO mice were less sensitive and NPY overexpressing mice were more sensitive to the sedative effects of alcohol, suggesting that decreased NPY signaling increases tolerance to alcohol intoxication, which could increase drinking (Thiele et al., 1998)