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To identify potential protein biomarkers associated with a disease status, plasma was collected from two independent Congolese cohorts, a discovery cohort (n = 60) and validation cohort (n = 204), sampled 10 years apart and subjected to multiple high-throughput assays

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To ensure a robust and representative evaluation of the assays accuracy and reliability, enzyme samples were prepared through serial dilutions, yielding glutathione peroxidase (GPx) activity levels that spanned a broad range from 50 to 500 U/l, allowing for a direct paired comparison