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Pharmacokinetic endpoints: if the research objective includes PK characterisation, build in LC-MS quantification of serum peptide at multiple timepoints to generate a concentration-time curve

To investigate whether GCG, VGF, SCG2, SCG3, and CHGA contribute to hyperglucagonemia in individuals with diabetes and diabetic mice, we examined gene expression data from cells in patients with type 2 diabetes (GSE84133) 42 and high-fat diet (HFD)-induced obese mice (GSE203151) 43

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274 A phase 2 study testing semaglutide enrolled 320 patients with MASH and fibrosis and randomized them to 0.1, 0.2, or 0.4 mg semaglutide group or the placebo group