The FDAs concern is twofold: Safety: Some substances are linked to toxicity, cardiac complications, or other serious adverse events
Troubleshooting involves verifying vendor quality, adjusting doses, extending protocol duration, and considering medical evaluation for underlying issues
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The cells were incubated at room temperature for 30 minutes in the dark, and the cell cycle was analyzed by flow cytometry (Win Bryte HS cytometer [Bio-Rad], using software Win Bryte, Bio-Rad Laboratories Inc., Hercules, CA, USA)
