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The selected amino acid site in the nanobody was replaced with a UAG stop codon by site-directed mutagenesis for incorporating ncAA with corresponding primers (Table S5)

We cannot exclude the possibility that loss of fluorescence during AITC treatment was due to its interference with the biomarker, but it has been reported that exogenously supplied chemicals responsible for GSH depletion are not likely to interfere with roGFP2 52

Fmoc-Leu-OH1-5 The molar number of Fmoc-Leu-OH is 1-5 times that of the resin

Genomic and functional characterization of the oas gene family encoding O-acetylserine (thiol) lyases, enzymes catalyzing the final step in cysteine biosynthesis in Arabidopsis thaliana

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