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Circulating and locally produced IGF-1 mediates many of GHs downstream effects and also feeds back negatively on both the hypothalamus and pituitary to restrain further GH release

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Methyl chloroformate (MCF) derivatization and Gas Chromatography-Mass Spectrometry (GC-MS) analysis All prepared extracts were chemically modified to lower their boiling point by MCF derivatization, based on the method published in Nature protocols (36)

doi: Parmar, R.M