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Extracellular vesicles in cancer metabolism: implications for cancer diagnosis and treatment

For each transfection, 5 l of Lipofectamine 2000 (11668019, Invitrogen) was added to 100 l of OptiMEM (Gibco), vortexed briefly and incubated for 5 minutes at room temperature

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Inositol is a key substrate in glucose metabolism and is a second messenger in insulin action

It also acts as a cofactor for detoxification enzymes, including glutathione peroxidases, thus helping preserve cellular redox homeostasis