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Because rapamycin, which extends mouse lifespan 31 , can reverse age-related diastolic dysfunction and restore a more youthful metabolome in the aged myocardium 32 , we next evaluated metabolomic changes in Young, Old Con, and Old mAb female-treated hearts

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3.5 Myosin light chain kinase and cytoskeletal regulation Myosin light chain kinase (MLCK)mediated regulation of epithelial barrier function is primarily controlled by coordinated Ca 2+ /calmodulin and RhoA/Rho-associated kinase (ROCK) signalling pathways that converge on phosphorylation of the regulatory myosin light chain (MLC-2), a central determinant of actomyosin contractility and TJ permeability (Cunningham and Turner, 2012)

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Scale bar, 10 m.( e-f ) Representative images ( e ) and quantification ( f ) of organoid clonogenicity from crypts isolated from WT and PPAR-3KO mice on CD or KD