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Cells were washed twice with PBS (without Ca 2+ and Mg 2+ ) and centrifuged at 600 g for 5 minutes at 4 C

IGF-1 exerts its biological effect through binding to the IGF1 receptor (IGF1R), its effect is modulated through interaction with a group of binding proteins (IGFBPs), which tend to inhibit the effect of IGF-1 [2]

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In patients with low-grade gliomas and patients with secondary glioblastoma, the proportion of IDH1 mutations can reach about 70%, but there is currently no selective treatment for IDH1 mutant gliomas

Crucial in preventing oxidative cell damage Reduced glutathione (GSH) has over 90% of the total glutathione pool in cells