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400 L supernatant was then vacuum concentrated to dryness, and the residue was re-dissolved in 80 L of 70% methanol solution, vortexed for 5 min, centrifuged at 18,000 rpm for 10 min, and the supernatant was used for analysis

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The classical somatomedin hypothesis (Salmon and Daughaday, 1957, foundational sulfation factor research) assumed all peripheral IGF-1 came from this hepatic source

The nervous system data is thin compared to the wound healing research, but it keeps this peptide on the radar for neuroscience-adjacent work