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Slices were then incubated overnight (1 slice/well) in 1 ml oxygenated Williams E Medium supplemented with 1 GlutaMax supplement, 25 mM D-glucose and 50 g/ml gentamicin at 37 C, using a Certomat CT Plus incubator at a shaking frequency of 80 rpm and continuous carbogen flow

Daniel Chille Personal Health Journey: Chronic gut issues revealed gaps in conventional care, emphasizing root cause medicine over symptom management

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Arnaoutoglou NA, OBrien JT, Underwood BR

To assess cell viability, 10 l CellTiter-Fluor (Promega) was added to black plates and incubated for 30 min in the dark before fluorescence was measured at 7-mm depth (gain 100)