Culture and differentiation of primary human bronchial epithelial cells (phBEC) Normal primary human bronchial epithelial cells (phBEC) from three healthy donors were obtained from Lonza (Wokingham, UK), cultured in BEGM medium (Lonza) and differentiated at the air-liquid interface (ALI) in PneumaCult-ALI medium (Stemcell Technologies, Cologne, Germany) for up to 2833 days as described previously 67
Providers must weigh unknown infant exposure risks against maternal health benefits when counseling breastfeeding mothers
H 2 S is mainly generated by three endogenous enzymes with different organ/tissue distributions, all of which are related to cysteine metabolism: the trans-sulphuration pathway pyridoxal-5-phosphate-dependent-CBS and CSE, and 3-MST
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SulfoCy5 either transitioned back from the dark state naturally, or low power 405 nm excitation (from 0.1 to 0.5 mW) was used to elicit the transition
