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TCGA database analysis revealed that patients with high GPX4 mRNA levels had a poorer prognosis than those in the group with low levels ( Figure 6B )

The scoring standard for IHC staining adopted the immunoreactive score (IRS) system [15, 16], with the specific rules as follows: the staining intensity of GGCT protein was classified into four grades, where a score of 0 indicated no staining signal, 1 indicated weak positivity, 2 indicated moderate positivity, and 3 indicated strong positivity

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