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In the rat model of IR injury, within 3 days, it was detected that retinal Fe 2+ and the oxidative stress markers, ROS and MDA, increased rapidly and reached the maximum peak at 48 h

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Notably, no differences were observed in NeuN-positive areas or cell numbers across all groups (Supplementary Fig

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Therefore, low glutathione levels can contribute to the inefficiency of methylation, creating a vicious cycle and furthering depleting ones glutathione levels