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SEC was performed by loading the protein onto Superose 6 Increase 10/300GL (GE Healthcare) column with running buffer containing 20 mM HEPES, pH 7.4, 100 mM NaCl, 10 mM MgCl 2 , 100 M TCEP, 5 M tirzepatide, 0.00075% (w/v) LMNG, 0.00025% (w/v) GDN, 0.0002% (w/v) CHS and 0.00025% digitonin (Anatrace)

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men typically need higher dosing per area due to greater muscle mass, and goals usually emphasise natural-looking results that preserve expression Patients seeking revision or complication management from previous nurse-led treatment elsewhere Not appropriate Pregnancy or breastfeeding Neuromuscular conditions (myasthenia gravis, Lambert-Eaton syndrome, motor neurone disease) Concurrent aminoglycoside antibiotics or certain other medications affecting neuromuscular junction Active infection or skin condition at the injection site Known hypersensitivity to botulinum toxin Patients with unrealistic expectations particularly those expecting deep static lines to disappear, sagging skin to be lifted, or volume to be restored Patients with body dysmorphia referred for psychological assessment first Patients under 18 (clinic policy) What well tell you at consultation We tell you directly if anti wrinkle injections are not the right treatment for your specific concern

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All three provide adequate subcutaneous tissue for proper absorption in most body types